And so an entirely new level of bronyhood is born.  Seriously, spread this thing around.  I'm making Pinkie Pie vs. Fluttershy next.

Backtrack is "Come From the Heart" by Skrillex.

LYRICS:

Twilight: Yo, my name is Twilight Sparkle, I mean serious business
I'm studying friendship from New Year's to Christmas
I live in a library, I know everything
About magic, leadership, literature, and astronomy
Check out my Cutie Mark, it's one of a kind
And I know the colts like it 'cause my plot's real fine
But hey, I don't strut it -- that'd be awful
'Cause you look like a pony who came out of a brothel
*chuckle* How you gonna battle to THAT, Trixie?

Trixie: Like THIS!
I am the Great and Powerful Trixie, so back off
Equestria Daily chose me as their mascot
All the colts want me, they even serve me luncheon
And unlike you, I don't have erectile dysfunction
See all these stars on my cape and hat?
Each one represents a time I've been in bed, take that!
I take on Ursa Majors, you only handle Minors
The G.&P.T. is by far the best rhymer

Twilight: Shut up, Trixie, no one cares for your bluffing
I'll run a marathon and leave you huffing and puffing
And that's a fact!  You better run back to Hoofington
Ponyville's mine!  There's no place for your overdone
Theatrical performances and hammy displays
I'm the princess's apprentice, and I'll school you anyday!
And guess what?  I'm voiced by Tara Strong, I'm empowered
You appeared in one episode and then ran like a coward

Trixie: You dare challenge me?  Trixie's taking out the trash!
You would've failed in magic school if it weren't for Rainbow Dash!
You're naive, clumsy, weak and flimsy, stubborn, clueless, makes me dizzy
How totally plain you look!  Get your head out of the books!
I'm shooting lightning, making storms, twisting rope while I perform
Been winning hearts since I was born, but you're stuck studying in your dorm
I travel Equestria coast to coast, I'm dominating the 4chan posts
You might bust boasts, but I'm far from toast, watch in awe as I turn you into a ghost!